expression of recombinant alpha-1 antitrypsin in cho and cos-7 cell lines using lentiviral vector

in this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (aat) with correct post-translational modifications, a protein production system based on the transduction of cho and cos-7 cells using lentiviral vectors was developed. human aat cdna was cloned into a replication-defective lentiviral vector. the transgene aat-jred chimer was transferred to cho and cos-7 cell lines using this vector and its expressions were visualized by fluorescent microscopy. the mrna expression levels of the aat genes were determined using revearse transcriptase-polymerase chain reaction (rt-pcr) and its secretion into the medium by both cell types was determined using elisa. the results show that by employing a lentiviral vector, efficient genetic loading of cho and cos-7 cells with the aat gene was achieved. in conclusion, by using a lentivirus-based gene delivery system, large amounts of recombinant human aat protein were expressed in both cho and cos-7 cell lines. this expression system possesses key properties that ensure its application in the delivery of therapeutic genes into mammalian cultured cells.